Goat Anti-Rat IgG Secondary Antibody (HRP)

Cat: SSA005

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Overview
 

Description:

Goat polyclonal Secondary Antibody to Rat IgG (HRP).

Host

Goat

Target species:

Rat IgG

Specificity:

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG.

 

Has cross-reactivity with human IgG, mouse IgG, rabbit IgG (>10%), bovine IgG (2.5%), horse IgG (7.2%), guinea pig IgG (6.8%)

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Protected from prolonged exposure to light. Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt. Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purity:

Immunogen affinity purified

Purification notes:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Clonality:

Polyclonal

Isotype:

IgG

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.5-10 μg/mL

ELISA

0.01-0.5 μg/mL

WB

0.05-1 μg/mL (Colormetric: 0.1-1 μg/mL; Chemiluminescent: 0.05-1 μg/mL)

 IP

0.05-1 μg/mL

 

Other Proteins

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