Flow Cytometry (FCM) / FACS Products Center

For choosing suitable antibodies for Flow cytometry detection, you need to consider

Nature of sample Choosing a secondary antibody when need
Species of sample The antibodies for dual staining
Species of primary antibody host Fluorochrome and chromogen labels

Sino Biological Flow Cytometry Antibody Products Facts

All Produced in House 8-156 Fold Improvement in ELISA Detection Limit
All Generated against Active Proteins or Precise Epitope Majority>95% purity Some>99%
Second Generation of Rabbit Mabs Over 1000 kinds of Flow Cytometry Antibodies
About 900 Rabbit / Mouse Mabs  

Flow Cytometry Antibodies Hot Target Products

Human

Mouse

Rat & Ferret

Flow Cytometry Antibodies by Immunogen Species

Even if the proteins of different species with the same name, they may be evolutionarily related, belong to the same protein family, but they may have different sequences and tertiary structures. One antibody may most likely not recognize the antigen which is come from different species to the target antigen. To ensure accurate results, you should choose an antibody that has been raised against the same species your sample is from. The antibody may react with the same target protein from other species sharing sufficient amino acid sequence homology.

If your sample is not from one of the species listed in the datasheet, this means that the species has not been tested and we cannot demonstrate suitability. A prediction of cross-reactivity is made based on sequence similarity.

Flow Cytometry Antibodies by Host & Clone

The species the primary antibody is raised in should be different from the species of your sample. This is to avoid cross-reactivity of the secondary anti-immunoglobulin antibody with endogenous immunoglobulins in the sample.

For instance, if you are studying a mouse protein, choose a primary antibody that is raised in a different species. A primary antibody raised in rabbit will be an appropriate choice, followed by an anti-rabbit IgG secondary antibody conjugated to a detection molecule (enzyme, fluorochrome, biotin, etc.). This issue can be avoided if a conjugated primary antibody is used.

For techniques using samples that do not contain endogenous immunoglobulin (IgG), the choice of host species of the primary antibody is less critical. An example is western blotting of a cell lysate that is not expected to contain IgG. However, tissue lysates and tissue culture supernatants that contain serum will contain immunoglobulins. IgG will appear in western blots of reduced, denatured samples as bands at 50 and 25 kDa corresponding to the heavy and light chains of the IgG molecule.

Flow Cytometry Antibodies by Research Area

Flow Cytometry Antibodies in Typical Aplications

Apoptosis
Immunophenotyping

Flow Cytometry Antibodies by Fluorochromes

 
Antibody
Antibody
Flow Cytometry (FCM) / FACS Antibody
Flow Cytometry (FCM) / FACS Antibody Features+
- Verified by Multiple Cell Samples
- Verified by Antigen Blocking
- Verified by Non-conjugated Antibody Blocking
- Verified by Fc Receptor Blocking
- Comparable with Classic Clone
- Antibody Verified by WB, IF and IHC
- Excellent Performance in Sensitivity
- Verified Stability
Flow Cytometry (FCM) / FACS Products Center+
- Choose Antibody for Flow Cytometry (FCM) / FACS
- Nature of Sample
- Species of Sample
- Antibody Host & Clone
- Secondary Antibody
- Dual Staining
- Fluorochrome Selection
- Hot Target Product
- Human Target Antibody
- Mouse Target Antibody
- Rat & Others Target Antibody
- Rabbit Monoclonal Antibody
- Mouse Monoclonal Antibody
- Rabbit Polyclonal Antibody
- Flow Cytometry (FCM) /FACS | Research Area
- CD Molecular
- Biomarker
- Biological Target
- Diagnostic
- Cancer
- Receptor
- Cytokine
- Interleukin
- Stem Cell
- FITC Labeled Antibody
- PE Conjugated Antibody
- APC Conjugated Antibody
- PerCP Conjugated Antibody
- Non-Conjugated Antibody
Flow Cytometry (FCM) / FACS Technology Center+
- Flow Cytometry (FCM) /FACS Introduction
- Flow Cytometry (FCM) /FACS Protocol
- Flow Cytometry (FCM) /FACS | Tips
- Flow Cytometry (FCM) /FACS | FAQ
- Flow Cytometry (FCM) /FACS | PPT
- Flow Cytometry (FCM) /FACS | Troubleshooting