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Flow Cytometry (FCM) / FACS Antibody

Flow Cytometry Antibody Search
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Strictly Validated Antibodies
  • So far, there are 1000+ strictly validated antibodies developed in Sino Biological Inc. favorable for Flow Cytometry assay;
  • Covering different speciese: Human, Mouse, Rat...;
  • Second generation of rabbit monoclonal antibodies included;
  • Suitable in various reserch areas for FCM: analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations,etc.;

Strictly Validated Antibodies

Excellent Performance in Sensitivity

 

Verified High Stability

  Classic Clone SB Clone
Recommended Concentration
1:10
1:100
 
Conjugated Time 0
12 Month
24 Month
42 Month
So far, there are over 1000 strictly validated antibodies developed in Sino Biological Inc. favorable for Flow Cytometry assay.

Choose the Appropriate Antibodies

Flow Cytometry Antibodies Hot Products

• CD3 • CD14
• CD4 • CD19
• CD5 • CD20
• CD8 • CD25
• CD45 • CD28

Flow Cytometry Antibodies by Immunogen Species

• Human Target Antibody
• Mouse Target Antibody
• Rat & Others Target Antibody

Flow Cytometry Antibodies by Host & Clone

• Rabbit Monoclonal Antibody
• Mouse Monoclonal Antibody
• Rabbit Polyclonal Antibody

Flow Cytometry Antibodies by Research Area

• CD Molecular
• Cancer
• Biomarker • Receptor
• Biological Target • Cytokine
• Diagnostic • Interleukin
  • Stem Cell

Flow Cytometry Antibodies in Typical Aplications

• Apoptosis
•Immunophenotyping

Flow Cytometry Antibodies by Fluorochromes

• FITC (Green) (515-545 nm)
• PE (Yellow) (564-601 nm)
• APC (Red) (653-669 nm)
• PerCP (Red) (676 nm)
• Non-conjugated

Flow Cytometry Introduction

Flow Cytometry Protocol

Recommendations for Background Control

  • • Unlabeled cells as blank control
  • • Single color and FMO control
  • • Isotype control
  • • Negative cells control

Fluorochrome Selection

  • • Excitation and Emission
  • • Fluorochrome Selection

Flow Cytometry Tips

  • • Sample preparation
  • • Fluorescence staining

Flow Cytometry FAQ

  • • What fluorochromes can I use?
  • • What controls do I need?
  • • Do the cells need to be permeabilized?
  • • Do the samples need to be fixed?

Flow Cytometry PPT

  • • Introduction to Flow Cytometry
  • • Flow Cytometry Basic Training
  • • Principles and Application of Flow Cytometry PowerPoint

Flow Cytometry Troubleshooting

  • • No staining
  • • Weak staining
  • • Nonspecific staining
  • • Unexpected staining

Flow Cytometry / FACS Background

Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection and protein engineering.

請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”