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Flow Cytometry (FCM) / FACS Antibody Features

Strictly validated antibodies are the premise of good results obtained in Flow Cytometry or FACS. So far, there are about 1000 fully validated antibodies developed in Sino Biological Inc. favorable for Flow Cytometry assay. All of them are verified for high specificity, high sensitivity, precise tissue or subcellular localization, and especially, avoiding false positive signal induced by Fc receptors on the surface of certain cell types.

Verified by Multiple Cell Samples

PC-3 A549 Lymphocyte Hela
Antigen: Human CD147
Antibody: CD147 / EMMPRIN / Basigin Antibody(FITC), Rabbit MAb (10186-R125F)
  Cell: Hela, PC-3, A549, Lymphocyte
Flow Cyt: Use 10 µl for 10^6 cells
Instrument: BD FACSCalibur
Sino Biological Flow Cytometry Antibody Products Facts:
  • • All Produced in House
  • • All Generated against Active Proteins or Precise Epitope
  • • Second Generation of Rabbit Mabs
  • • About 900 Rabbit / Mouse Mabs
  • • 8-156 Fold Improvement in ELISA Detection Limit
  • • Majority>95% purity, Some>99%

Browse all Flow Cytometry Antibodies

Antigen Blocking Verified

Antigen: Human ICOSLG (CD275)
Antibody: ICOS Ligand / B7-H2 Antibody (FITC), Mouse MAb (11559-MM01)
Cell: RPMI8226
Flow Cyt: Use 1 µg for 10^6 cells
Instrument: BD FACSCalibur
Antigen: Human JAM-A (CD321)
Antibody: JAM-A / F11R Antibody, Mouse MAb (10198-MM04)
Cell: A431
Flow Cyt: Use 1 µg for 10^6 cells
Instrument: BD FACSCalibur

Non-conjugated Antibody Blocking Verified

Antigen: Human CD146
Antibody: CD146 / MCAM Antibody (FITC), Rabbit MAb (10115-R044-F)
Cell: HeLa
Flow Cyt: Use 10 µl for 10^6 cells
Instrument: BD FACSCalibur
Antigen: Human IGF1R
Antibody: IGF1R / CD221 Antibody (PE), Rabbit MAb(10164-R012-P)
Cell: HeLa
Flow Cyt: Use 5 µl for 10^6 cells
Instrument: BD FACSCalibur

Fc Receptor Blocking Verified

Antigen: Human CD177
Antibody: CD177 / PRV-1 / PRV1 Antibody (FITC), Mouse MAb (14501-MM05-F)
Cell:human whole blood granulocytes
Flow Cyt: Use 1µg for 10^6 cells
Instrument: BD FACSCalibur
Antigen: Mouse CD155
Antibody: CD155 / PVR Antibody, Rabbit MAb (50259-R305)
Cell: Mouse splenocytes
Flow Cyt: Use 1µg for 10^6 cells
Instrument: BD FACSCalibur

WB, IF and IHC Verified

Antigen: Human CD147
Antibody: CD147 / EMMPRIN / Basigin (10186-H08H)
Cell: A431
WB: 30 ug/lane
Recommended Concentration: 5-10 µg/ml
Secondary Antibody: Dylight 800-labelled Antibody to Rabbit IgG (H+L), at 1:5000 Dilution
Antigen: Human CD147
Antibody: CD147 / EMMPRIN / Basigin (10186-H08H)
Cell: HeLa
IF: 4% PFA in PBS, rt 15min, rabbit anti-CD147 monoclonal antibody (15 µg/ml) at 4℃ overnight, counterstained with DAPI (blue)
Recommended Concentration: 5 µg/ml Secondary Antibody: Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG (green), 1:500
Instrument: Nikon 80i
Antigen: Human CD147
Antibody: CD147 / EMMPRIN / Basigin (10186-H08H)
Cell: Human gastric cancer epithelium cell
IHC: 0.2 µg/mL, formalin-fixed paraffin embedded sections, antigen retrieval with citrate buffer (pH 6.0), micro-oven 15 min
Recommended Concentration: 0.1-5 μg/ml
Instrument: Nikong 80i,200×

Comparable to Classic Clone

Antigen: Human CD3E
Antibody: CD3e / CD3 epsilon Antibody (FITC), Mouse MAb (10977-M001-F); CD3e / CD3 epsilon Antibody (APC), Mouse MAb (10977-M001-A); CD3e / CD3 epsilon Antibody (PE), Mouse MAb (10977-M001-P)
Cell: Human whole blood lymphocytes
Flow Cyt: Use 1µl for 10^6 cells
Instrument: BD FACSCalibur
  SBI CD3  

Excellent Performance in Sensitivity

  Recommended Concentration 1:10 1:100  
BD CD14 Antigen: Human CD14
Antibody: CD14 Antibody (FITC), Mouse MAb (10073-MM06-F)
Cell: Human whole blood monocytes
Flow Cyt: Use 10µl for 10^6 cells
Instrument: BD FACSCalibur
SBI Human CD14-MM06H  

Verified High Stability

Antigen: Human E-CAD
Antibody: E-Cadherin / CDH1 / E-cad / CD324 Antibody (PE), Mouse MAb(10204-MM08-P)
Cell: MCF-7
Flow Cyt: Use 5 µl for 10^6 cells
Instrument: BD FACSCalibur
Antigen: Human EGFR
Antibody: EGFR / HER1 / ErbB1 Antibody (FITC), Rabbit MAb(10204-MM08-P)
Cell: A431
Flow Cyt: Use 5 µl for 10^6 cells
Instrument: BD FACSCalibur
請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”