DYKDDDDK peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti- DYKDDDDK tag magnetic beads. Use DYKDDDDK peptide to elute DYKDDDDK-tagged proteins. Elution yields will increase by incubating the peptide on the tube for 10-15 minutes at 30-37 oC for each elution fraction.
1. Prepare DYKDDDDK peptide at 1mg/mL in PBS.
2. Add 50 μL 1mg/mL DYKDDDDK peptide to the protein combined beads (initially adding 50 μL Sino Biological DYKDDDDK tag magnetic beads, TB101274), gently vortex to mix and incubate the sample at 37 ℃ on a rotator for 5-10 minutes. Elution may be performed at reduced temperatures, but lower yields result.
3. Separate the beads on a magnetic separator and save the supernatant containing the target antigen.
4. Repeat elution step once for higher recovery.
|Immunoprecipitation Kit -DYKDDDDK（Flag®）Tag Immunomagnetic Beads||TB101274|