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DYKDDDDK Synthetic Peptide

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    Product Introduction
     

    DYKDDDDK peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti- DYKDDDDK tag magnetic beads. Use DYKDDDDK peptide to elute DYKDDDDK-tagged proteins. Elution yields will increase by incubating the peptide on the tube for 10-15 minutes at 30-37 oC for each elution fraction.

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    FLAG Synthetic Peptide
     

    1. Prepare DYKDDDDK peptide at 1mg/mL in PBS.
    2. Add 50 μL 1mg/mL DYKDDDDK peptide to the protein combined beads (initially adding 50 μL Sino Biological DYKDDDDK tag magnetic beads, TB101274), gently vortex to mix and incubate the sample at 37 ℃ on a rotator for 5-10 minutes. Elution may be performed at reduced temperatures, but lower yields result.
    3. Separate the beads on a magnetic separator and save the supernatant containing the target antigen.
    4. Repeat elution step once for higher recovery.

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    Immunoprecipitation Kit -DYKDDDDK(Flag®)Tag Immunomagnetic Beads TB101274
    Size / Price
    貨號: PP101274-5
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