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Expression-ready ORF Clone

Multiple species: Human, mouse, rat, monkey, canine, ferret and virus.
Wide variety of tags to choice:untagged,C-or N-terminal,Myc, HA, FLAG,
His,GFPSpark® and OFPSpark®
High protein expression level: enhanced CMV promoter
Only ORF sequences,no 5’ or 3’UTRs
Designed for mammalian expression, stable or transient
Fully sequence confirmed
Expression validated

Expression-ready ORF Clone: Higher protein expression level

The plasmid was transfected into mammalian cells with Sinofection reagent (Cat#STF02) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. EGFP protein expression levels were significantly increased in different cell lines.

pCMV3-EGFP(Sinobiological)

pCDNA3.1-EGFP( other company)

Expression-ready ORF Clone: Expression validated

Sinobiological provide ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately.Now thousands of molecules have been expression validated.
   
The Myc,HA,FLAG,His tagged ORF clones were expression validated by IF (Immunofluorescence). The plasmids were transfected into mammalian cells. After 48h, cells were fixed and blocked, incubated with primary antibodies, then stained with secondary antibody. Finally the fluorescent signals can be detected by fluorescence microscope.
   
The GFPSpark® & OFPSpark® tagged ORF clones were transfected into cells transiently. After 48h, the fluorescent signals can be detected by fluorescence microscopy.

Partly expression validated image

Catalog No. Gene Description Image Shopping
HG10304-NF PTPN1 Human PTP1B / PTPN1 Gene ORF cDNA clone expression plasmid, N-Flag tag in stock
HG11430-ACR PKM Human PKM2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag in stock
HG11508-ACR MTOR Human MTOR Gene ORF cDNA clone expression plasmid, C-OFPSpark tag in stock
HG12054-ACG RELA Human RELA / Transcription factor p65 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag in stock
HG12055-ANG IDH1 Human IDH1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tag in stock
HG12125-NF FTO Human Fat mass and obesity associated/FTO Gene ORF cDNA clone expression plasmid, N-Flag tag in stock
HG12156-ACG SIRT6 Human SIRT6 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag in stock
HG12283-CF CD47 Human CD47 Gene ORF cDNA clone expression plasmid, C-Flag tag in stock
MG50843-CF CXCR5 Mouse CXCR5 Gene ORF cDNA clone expression plasmid, C-Flag tag in stock
RG80100-NF EGFR Rat EGFR/HER1 Gene ORF cDNA clone expression plasmid, N-Flag tag in stock
VG40042-CF RSV-F Human respiratory syncytial virus (RSV) (subtype A, strain A2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-Flag tag in stock
VG40110-CF H7N9-NP Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag in stock

See more expression validated ORF Clone

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Organelle Marker Plasmid

Sinobiological provide a collection of organelle marker plasmids which encoding organelle-specific or structure-specific proteins. The proteins are fused with GFPSpark® or OFPSpark® tag and allow direct visualization of the organelles or structures without manipulation by fluorescence microscopy.Organelle marker plasmids represent a part of expression ORF clone. Learn more about........

Microtuble Mitochondrion Golgi apparatus Mitochondria Endosome Endoplasmic Reticulum
human TUBB
HG11626-ACG
human TOMM20
HG15094-ACG
human GOLM1
HG13066-ACG
human PRDX5
HG11263-ACR
human RAB5A
HG14013-ANR
human HSPA5
HG12063-ACR

The GFPSpark® & OFPSpark® tag ORF clones were transfected into Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope(Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).

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請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”