Expression-ready ORF Clone

Sino Biological provides ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately. Now thousands of molecules have been expression validated. Sino Biological has always been committed to providing customers with high quality products and services.

Advantages of Expression-ready ORF Clone

Multiple Species   Expression Validated
Human, mouse, rat, monkey, canine, ferret and virus... Expression validated, high expression level
Multiple Tags Applicability
• Untagged
• C-terminal:Myc, HA, FLAG, His, GFPSpark® and OFPSpark®
• N-terminal:Myc, HA, FLAG, His, GFPSpark® and OFPSpark®
Designed for mammalian expression, stable or transient
Full Length Sequence Confirmed High Expression Level
Full length sequence confirmed, quality assurance Enhanced CMV promoter

Expression-ready ORF Clone: Higher protein expression level

The plasmid was transfected into mammalian cells with Sinofection reagent (Cat#STF02) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. EGFP protein expression levels were significantly increased in different cell lines.

• pCMV3-EGFP(Sino Biological):

• pCDNA3.1-EGFP(Other company):

Expression-ready ORF Clone: Expression validated

Sino Biological provides ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately. Now thousands of molecules have been expression validated.
The Myc, HA, FLAG, His tagged ORF clones were expression validated by IF (Immunofluorescence). The plasmids were transfected into mammalian cells. After 48h, cells were fixed and blocked, incubated with primary antibodies, then stained with secondary antibody. Finally the fluorescent signals can be detected by fluorescence microscope.
The GFPSpark® & OFPSpark® tagged ORF clones were transfected into cells transiently. After 48h, the fluorescent signals can be detected by fluorescence microscopy.
Human PTPN1,N-Flag
HG10304-NF
Human IDH1,N-GFPSpark®
HG12055-ANG
Human SIRT6,C-GFPSpark®
HG12156-ACG
H7N9-NP,C-Flag
VG40110-CF
 
   
Human PKM,C-OFPSpark®
HG11430-ACR
Human MTOR,C-OFPSpark®
HG11508-ACR
   

Expression-ready ORF Clone by Tags

• Untagged cDNA ORF Clone • N / C-FLAG Tag cDNA ORF Clone
• N / C-Myc Tag cDNA ORF Clone • N / C-His Tag cDNA ORF Clone
• N / C-HA Tag cDNA ORF Clone • N / C-GFPSpark® Tag cDNA ORF Clone
• N / C-OFPSpark® Tag cDNA ORF Clone  

Partly Expression Validated ORF Clones

Catalog No. Molecule Product Description Availability
HG10304-NF PTPN1 Human PTP1B / PTPN1 Gene ORF cDNA clone expression plasmid, N-Flag tag In stock
HG11430-ACR PKM Human PKM2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag In stock
HG11508-ACR MTOR Human MTOR Gene ORF cDNA clone expression plasmid, C-OFPSpark tag In stock
HG12054-ACG RELA Human RELA / Transcription factor p65 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag In stock
HG12055-ANG IDH1 Human IDH1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tag In stock
HG12125-NF FTO Human Fat mass and obesity associated/FTO Gene ORF cDNA clone expression plasmid, N-Flag tag In stock
HG12156-ACG SIRT6 Human SIRT6 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag In stock
HG12283-CF CD47 Human CD47 Gene ORF cDNA clone expression plasmid, C-Flag tag In stock
MG50843-CF CXCR5 Mouse CXCR5 Gene ORF cDNA clone expression plasmid, C-Flag tag In stock
RG80100-NF EGFR Rat EGFR/HER1 Gene ORF cDNA clone expression plasmid, N-Flag tag In stock
VG40042-CF RSV-F Human respiratory syncytial virus (RSV) (subtype A, strain A2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-Flag tag In stock
VG40110-CF H7N9-NP Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag In stock

Organelle Marker Plasmid-GFPSpark® /OFPSpark® tagged-expression ready ORF Clones

Sino Biological provides a collection of organelle marker plasmids which encoding organelle-specific or structure-specific proteins. The proteins are fused with GFPSpark® or OFPSpark® tag and allow direct visualization of the organelles or structures without manipulation by fluorescence microscopy. Organelle marker plasmids represent a part of expression ORF clone. Learn more about...
The GFPSpark® & OFPSpark® tag ORF clones were transfected into Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope (Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).

Microtuble Mitochondria Golgi apparatus Autophagosome
Human TUBB
HG11626-ACG
Human TOMM20
HG15094-ACG
Human GOLM1
HG13066-ACG
Human LC3B
HG14555-ACG
 
Cytoskeleton Mitochondria Endosome Endoplasmic Reticulum
Human EB3
HG14614-ACR
Human PRDX5
HG11263-ACR
Human RAB5A
HG14013-ANR
Human HSPA5
HG12063-ACR

Mammalian Expression Vectors

• pCMV3-C-Flag • pCMV3-N-Flag • pCMV3-Untagged
• pCMV3-C-HA • pCMV3-N-HA • pCMV3-SP-N-Flag
• pCMV3-C-Myc • pCMV3-N-Myc • pCMV3-SP-N-HA
• pCMV3-C-His • pCMV3-N-His • pCMV3-SP-N-Myc
• pCMV3-C-OFPSpark® • pCMV3-N-OFPSpark® • pCMV3-SP-N-His
• pCMV3-C-GFPSpark® • pCMV3-N-GFPSpark®  

Other Vectors In Sino Biological

Molecular Biology Service
• Molecular Biology Service
• Real Time PCR Service
• Real Time PCR Primer and Probe Design
• Real Time Quantitative PCR Analysis