ELISA kit troubleshooting

Though without that complicated protocols as IHC, immunoprecipitation etc, ELISA assay might generate many problems as what could occur in most of the immuno assays. This part provides troubleshooting of ELISA kit with means of cause-solution table with problems listed beforehand. Please note that the conditions described here may not pertain to every ELISA kit because performance requirements vary for individual assays. Be sure to check your package insert for specifications.

No Signal
Poor
Poor
Detection
Value
Non
-Specificity
High
Background

As below we lists the most common problems in ELISA assay, click for more details.

Antibody
Antibody
ELISA Antibody
ELISA KIT + ELISA Pair set
ELISA KIT+
- ELISA Kit features
- ELISA Kit product center
- ELISA Kit technology center
ELISA kit Introduction
What is being tested using ELISA Kit?
What is ELISA kit Mechanism
ELISA kit Advantages
ELISA Kit principle
ELISA kit protocol
ELISA kit troubleshooting
ELISA kit Troubleshooting-No signal
ELISA kit Troubleshooting-Poor Standard Curve
ELISA kit Troubleshooting-Poor detection value
ELISA kit Troubleshooting-High Background
ELISA kit Troubleshooting-Non-specificity
ELISA kit FAQ
ELISA kit Tips
ELISA kit storage
ELISA kit material provided
ELISA Kit precaution and safety instructions
ELISA Kit Terms
ELISA Kit term-Solid phase
ELISA Kit term-Adsorption
ELISA Kit term-Washing
ELISA Kit term-Enzyme
ELISA Kit term-Enzyme conjugate
ELISA Kit term-Substrate
ELISA Kit term-Chromophore
ELISA Kit term-Stopping
ELISA Kit term-Reading
ELISA Kit term-linearity range
ELISA Kit term-Limit of detection
ELISA Kit term-Precision
ELISA Kit term-Linearity
ELISA Kit term-Recovery
ELISA Kit term-Stability
ELISA Kit term-Interfering test
ELISA Kit term-Cross reaction
ELISA Kit term-Natural samples Detection