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ELISA kit Troubleshooting - Non-specificity

Though without that complicated protocols as IHC, immunoprecipitation etc, ELISA assay might generate many problems as what could occur in most of the immuno assays. This part provides troubleshooting of ELISA kit with means of cause-solution table with problems listed beforehand. Please note that the conditions described here may not pertain to every ELISA kit because performance requirements vary for individual assays. Be sure to check your package insert for specifications.

This section is intended to recommend possible causes and relative troubleshooting for the problem of ELISA poor detection value.

Possible Sources Solutions
Samples were contaminated Avoid cross contamination of samples
The concentration of samples was too high Try higher dilution rate of samples