ELISA kit Troubleshooting-No signal

Though without that complicated protocols as IHC, immunoprecipitation etc, ELISA assay might generate many problems as what could occur in most of the immuno assays. This part provides troubleshooting of ELISA kit with means of cause-solution table with problems listed beforehand. Please note that the conditions described here may not pertain to every ELISA kit because performance requirements vary for individual assays. Be sure to check your package insert for specifications.

No Signal
Poor
standard
curve
Poor
Detection
Value
Non
-Specificity
High
Background
Possible Sources Solutions
Incorrect or no detection antibody was added Add appropriate detection antibody and continue
Substrate solution was not added Add substrate solution and continue
Incorrect storage condition Check if the kit is stored at recommended condition and used before expiration date
Antibody
Antibody
ELISA Antibody
ELISA KIT + ELISA Pair set
ELISA KIT+
- ELISA Kit technology center
ELISA kit Introduction
ELISA Kit principle
ELISA kit protocol
ELISA kit troubleshooting
ELISA kit Troubleshooting-No signal
ELISA kit Troubleshooting - Poor Standard Curve
ELISA kit Troubleshooting - Poor detection value
ELISA kit Troubleshooting - High Background
ELISA kit Troubleshooting - Non-specificity
ELISA kit FAQ
ELISA kit Tips
ELISA kit storage
ELISA kit material provided
ELISA Kit precaution and safety instructions
ELISA Kit Terms