ELISA Kit principle

The ELISA method is a benchmark for quantitation of pathological antigens and there are indeed many variations to this method. ELISAs are adaptable to high-throughput screening because results are rapid, consistent and relatively easy to analyze. The best results have been obtained with the sandwich format, utilizing highly purified, prematched capture and detector antibodies. The resulting signal provides data which is very sensitive and highly specific.

The principle of Sinobiological Inc. ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. The capture antibody is pre-coated onto well plate strips. Standards and samples are added to the wells and target protein present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-target protein antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of target protein bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).

ELISA Antibody
- ELISA Kit features
- ELISA Kit product center
- ELISA Kit technology center
ELISA kit Introduction
What is being tested using ELISA Kit?
What is ELISA kit Mechanism
ELISA kit Advantages
ELISA Kit principle
ELISA kit protocol
ELISA kit troubleshooting
ELISA kit Troubleshooting-No signal
ELISA kit Troubleshooting-Poor Standard Curve
ELISA kit Troubleshooting-Poor detection value
ELISA kit Troubleshooting-High Background
ELISA kit Troubleshooting-Non-specificity
ELISA kit Tips
ELISA kit storage
ELISA kit material provided
ELISA Kit precaution and safety instructions
ELISA Kit Terms
ELISA Kit term-Solid phase
ELISA Kit term-Adsorption
ELISA Kit term-Washing
ELISA Kit term-Enzyme
ELISA Kit term-Enzyme conjugate
ELISA Kit term-Substrate
ELISA Kit term-Chromophore
ELISA Kit term-Stopping
ELISA Kit term-Reading
ELISA Kit term-linearity range
ELISA Kit term-Limit of detection
ELISA Kit term-Precision
ELISA Kit term-Linearity
ELISA Kit term-Recovery
ELISA Kit term-Stability
ELISA Kit term-Interfering test
ELISA Kit term-Cross reaction
ELISA Kit term-Natural samples Detection