ELISA KIT + ELISA Pair set

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Human AFP / alpha-fetoprotein ELISA Kit Human BMP-2 ELISA Kit
Human Complement Component C2 ELISA Kit Human CD38 ELISA Kit
Human CD40 / TNFRSF5 ELISA Kit Human IL6 / IL-6 ELISA Kit
Human Cystatin B / CSTB ELISA Kit Human CXCL9 / MIG ELISA Kit
Mouse DKK3 ELISA Kit Human DLL1 / Delta-like 1 ELISA Kit
Human IL-1 beta / IL1B / IL-1F2 ELISA Pair Set Human IL-15 / IL15 / Interleukin 15 ELISA Pair Set
Human IL6 / Interleukin-6 / IFNB2 ELISA Pair Set Human IL-16 ELISA Pair Set
Human IL-20 / Interleukin-20 ELISA Pair Set Human IL-5 / Interleukin 5 ELISA Pair Set
Mouse IL-16 / Interleukin-16 ELISA Pair Set Human IL12B / NKSF2 / p40 ELISA Pair Set
Human IL-6 / Interleukin-6 ELISA Pair Set Human IL-12 (IL12A & IL12B Heterodimer) ELISA Pair Set

ELISAs Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries,such as ELISA application in food industry. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate, see in detail in the section of ELISA device) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. The part of antibody incubation of ELISA is similar with that of western blot. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

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Antibody
ELISA Antibody
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ELISA KIT + ELISA Pair set
- ELISA KIT
- ELISA Pair Set
ELISA Antibody Immunoprecipitation / IP Antibody Immunohistochemistry / IHC Antibody
Flow Cytometry (FCM) / FACS Antibody Western blotting / WB Antibody Immunofluorescence / IF / ICC Antibody
Tag Antibodies Secondary Antibodies Control Antibodies
Loading Control antibodies Mouse Mab Rabbit Mab
Rabbit Pab Biomarker Antibody CD Molecule Antibody
Stem Cell Research Antibody Receptor Antibody Interleukin Antibody
Cytokine Antibody Biological Target Antibody Diagnostic Antibody
Cancer Antibody