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ELISA Antibody-Rabbit Polyclonal Antibody

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Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

If an immunogen is injected into an animal, a number of cells will bind that antigen, so the antibody which appears in the bloodstream. The antibodies will have arisen from several clones of cells and are different, so the antibody in bloodstream is a polyclonal antibody. Polyclonal antibody contain different antibodies bind to different antigen.

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