E.coli Protein Expression Service

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E.coli Protein Expression Service Introduction

E.coli protein expression system is now the most wide-used and economical expression system. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle, strong anti-pollution ability and easy to be scaled up. E.coli protein expression system is suitable for protein expression of multiple species, acting as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small protein production. With abundant experience of soluble protein expression and purification and protein refolding, and multiple E.coli cell lines and expression vectors, Sino Biological can provide you high-quality expression service.

E.coli Protein Expression Service Advantages

Over 10 years' experience of protein expression
Multiple protein purification and refolding technologies
High-throughput and large-scale protein expression and production
One-stop service from gene to purified protein
Multiple expression vectors and E.coli cells to meet various expression needs

E.coli Protein Expression Service Procedures

Communicate about protein expression requirements  Sign contract and make prepayment  Carry out pilot study for protein expression  Feedback pilot study result and ship sample (if feasible)  Bulk production quotation  Bulk production based on customer requirements

E.coli Protein Expression Service Items

Service procedures and items Cycle Delivery
Gene synthesis and codon optimization (optional) 1-2 weeks  
Vector construction 1-2 weeks Sequencing report
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
Expression and purification 1-2 weeks • Feasibility report
• 1-3 mg sample (if expression is feasible and sample remains from QC testing)
• transformation into proper strain
• pilot scale expression and condition optimization
• affinity purification (supernatant)
• refolding (inclusion body)
• SDS-PAGE and UV analysis
Large-scale expression and purification (optional) 2-3 weeks • Purified protein
• SDS-PAGE report
• CoA