Anti-Cofilin 2 Antibody (Rabbit Polyclonal antibody) General Information
Anti-Cofilin 2 Antibody
Reacts with: Human
Predicted to React with:
Mouse (Species predicted to react based on 100% sequence homology)
Human Cofilin 2
A synthetic peptide corresponding to the C-terminus of the Human cofilin 2 / CFL2
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human cofilin 2 / CFL2, and purified by antigen affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cofilin 2 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Cofilin 2 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of human CFL2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of CFL2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CFL2 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Anti-Cofilin 2 Antibody Alternative Names
Cofilin 2 Background Information
Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.
cofilin 2 (muscle)
Aizawa H, et al. (2001) Cofilin-2, a novel type of cofilin, is expressed specifically at aggregation stage of Dictyostelium discoideum development. Genes Cells. 6 (10): 913-21.Papalouka V, et al. (2009) Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Papalouka V, Mol Cell Biol. 29 (22): 6046-58.