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CD59 cDNA ORF Clone, Human, C-GFPSpark® tag

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CD59 cDNA ORF Clone, Human, C-GFPSpark® tag: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
1115 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human CD59 molecule, complement regulatory protein with C terminal GFPSpark tag.
Plasmid
Promoter
Enhanced CMV promoter
Restriction Sites
KpnI + XbaI(6kb+1.12kb)
Tag Sequence
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

CD59 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD59 cDNA ORF Clone, Human, C-GFPSpark® tag: Validated Images

CD59 cDNA ORF Clone, Human, C-GFPSpark® tag: Alternative Names

16.3A5 cDNA ORF Clone, Human; 1F5 cDNA ORF Clone, Human; EJ16 cDNA ORF Clone, Human; EJ30 cDNA ORF Clone, Human; EL32 cDNA ORF Clone, Human; FLJ38134 cDNA ORF Clone, Human; FLJ92039 cDNA ORF Clone, Human; G344 cDNA ORF Clone, Human; HRF-20 cDNA ORF Clone, Human; HRF20 cDNA ORF Clone, Human; MAC-IP cDNA ORF Clone, Human; MACIF cDNA ORF Clone, Human; MEM43 cDNA ORF Clone, Human; MGC2354 cDNA ORF Clone, Human; MIC11 cDNA ORF Clone, Human; MIN1 cDNA ORF Clone, Human; MIN2 cDNA ORF Clone, Human; MIN3 cDNA ORF Clone, Human; MIRL cDNA ORF Clone, Human; MSK21 cDNA ORF Clone, Human; p18-20 cDNA ORF Clone, Human

CD59 Background Information

CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Full Name
CD59 molecule
References
  • Fletcher CM. et al., 1994, Structure. 2: 185-99.
  • Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44.
  • Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81.
  • Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
  • Picariello G. et al., 2008, Proteomics 8: 3833-47.
  • Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
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