Anti-CD31 Antibody

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Anti-CD31 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-CD31 Antibody
Validated applications
FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human CD31
No cross-reactivity in ELISA with
Human CD54
Human CD62
Human CD106
Human cell lysate (293 cell line)
Immunogen
Recombinant Human CD31 protein (Catalog#10148-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 extracellular domain (rhCD31; Catalog#10148-H08H; Met 1-Lys 601; NP_000433.3). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #13
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD31 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD31 Antibody (Mouse Monoclonal antibody) Images

Immunofluorescence staining of Human CD31 in HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD31 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody. Positive staining was localized to cell membrane.
Flow cytometric analysis of anti-human CD31 on human whole blood monocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable monocytes.

Anti-CD31 Antibody: Alternative Names

Anti-CD31 Antibody; Anti-CD31/EndoCAM Antibody; Anti-endoCAM Antibody; Anti-GPIIA' Antibody; Anti-PECA1 Antibody; Anti-PECAM-1 Antibody

CD31 Background Information

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 13-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
Full Name
platelet/endothelial cell adhesion molecule 1
References
  • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
  • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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