TIM-1 / KIM-1 ELISA Kit, Canine

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TIM-1 / KIM-1 ELISA Kit, Canine: General Information

Product name
TIM-1 / KIM-1 ELISA Kit, Canine
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Urine
Sensitivity
69 pg/mL
Assay range
156.1-10000 pg/mL
Specificity
Recognizes both recombinant and natural Canine TIM-1 / KIM-1
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2.05 4.20 8.30
SD 0.10 0.17 0.37
CV(%) 4.8% 3.9% 4.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2.12 4.29 8.69
SD 0.14 0.24 0.42
CV(%) 6.5% 5.6% 4.8%
Recovery
The recovery of Canine HAVCR1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=0)
Average % Recovery
87
Range
85-89%
Linearity
Urine
  Recovery of detected
1:2 86%
1:4 90%
1:8 91%
1:16 85%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TIM-1 / KIM-1 ELISA Kit, Canine is an enzyme-linked immunosorbent assay for the quantitative measurement of Canine TIM-1 / KIM-1 protein in Urine . It contains recombinant Canine TIM-1 / KIM-1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TIM-1 / KIM-1 ELISA Kit, Canine: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural canine KIM-1. The factors listed below were prepared at 1000 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TIM-1 / KIM-1 Background Information

HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulinmucin 1 (TIM-1), is a type â… integral membrane glycoprotein. KIM-1 protein is widely expressed with highest levels in kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.
Full Name
hepatitis A virus cellular receptor 1
References
  • Tami C, et al. (2007) Immunoglobulin A (IgA) is a natural ligand of hepatitis A virus cellular receptor 1 (HAVCR1), and the association of IgA with HAVCR1 enhances virus-receptor interactions. J Virol. 81(7): 3437-46.
  • Rees AJ, et al. (2008) Kim-1/Tim-1: from biomarker to therapeutic target? Nephrol Dial Transplant. 23(11): 3394-6.
  • Chaturvedi S, et al. (2009) Assay validation for KIM-1: human urinary renal dysfunction biomarker. Int J Biol Sci. 5(2): 128-34.
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