A bispecific antibody is an artificial antibody containing two kinds of specific antigen binding sites and can establish a bridge between target cells and functional molecules (cells) to stimulate a directed immune response. It is a genetically engineered antibody, which has become a hot spot in the field of antibody engineering, and has a broad application prospect in tumor immunotherapy. Based on the optimized mature mammalian cell expression platform, Sino Biological can provide you with fast and efficient bispecific antibody expression service.
| Service procedures | Specification | Timeline | Deliverables | Pricing |
|---|---|---|---|---|
| ① Gene synthesis and codon optimization (optional) |
1-2 weeks | • Purified antibody • SDS-PAGE data • CoA |
Please fill in the form and send it to cro-service@sinobiological.com |
|
| ② Vector construction |
• Expression vector construction • Plasmid sequencing • Large scale plasmid production Expression & purification |
1-2 weeks | ||
| ③ Expression & purification |
• Transient transfection of HEK293 / CHO cells • Purify the antibody • Polishing if needed |
1-2 weeks | ||
| ④ QC analysis |
• Analysis by SDS-PAGE and UV • Purity analysis by HPLC-SEC and endotoxin level analysis (optional) |
3 days |
1.Client needs to provide the antibody sequence or plasmid.
2.A standard service includes: antibody concentration detected by A280 and purity evaluated by SDS-PAGE. If other detection services such as SEC-HPLC and endotoxin level test are required, please indicate it in the request form.
