beta-Actin Loading Control Antibody, Mouse MAb

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beta-Actin Loading Control Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
beta-Actin Loading Control Antibody, Mouse MAb
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human beta-Actin
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin / ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #10
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

beta-Actin Loading Control Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:2500-1:200000
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

beta-Actin Loading Control Antibody, Mouse MAb (Mouse Monoclonal antibody) Images

Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: NIH3T3 Whole Cell Lysate

Lane C: RAW264.7 Whole Cell Lysate

Lane D: Mouse slpeen tissue lysate

Lane E: Mouse lung tissue lysate

Lane F: Mouse brain tissue lysate

Lane G: C6 whole cell lysate

Lane H: PC-12 whole cell lysate

Lane I: Rat liver tissue lysate

Lane J: Rat lung tissue lysate

Lane K: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

HRP Conjugated Goat anti-Mouse IgG (H+L) ,at 1:10000 dilution .

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:44 kDa

Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lane D: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:44 kDa

ACTB was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ACTB mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ACTB mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution

Lysates/proteins per lane

HepG2 (30 μg), Hela (5 μg), Raw246.7 (30 μg)

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 43 kDa

Observed band size: 44 kDa

beta-Actin Loading Control Antibody, Mouse MAb: Alternative Names

Anti-BRWS1 Antibody; Anti-PS1TP5BP1 Antibody

beta-Actin Background Information

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.
Full Name
actin, beta

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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