Anti-beta-Actin Antibody

All beta-Actin Reagents

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) General Information

Product name

Anti-beta-Actin Antibody

Validated applications

WB,IP

Species reactivity

Reacts with: Human

Specificity

Human beta-Actin

Immunogen

A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB

Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB, and purified by antigen affinity chromatography.

Source

Polyclonal Rabbit IgG

Purification

Protein A & Antigen Affinity

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Validated Applications

Application	Dilution	Notes
WB	1:500-1:2000
IP	1-4 uL/mg of lysate

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Images

Human beta Actin/ACTB Immunoprecipitation(IP) 6113

ACTB was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-ACTB rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ACTB rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Human beta Actin/ACTB Western blot (WB) 6827

Anti-ACTB，beta-actin cytoplasmic1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: Mouse brain tissue lysate Whole Cell Lysate

Lane D: Mouse brain tissue lysate

Lane E: Rabbit brain tissue lysate

Lane F: Rabbite slpeen tissue lysate

Lane G: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:42 kDa

Anti-beta-Actin Antibody Alternative Names

Anti-BRWS1 Antibody;Anti-PS1TP5BP1 Antibody

beta-Actin Background Information

Full Name

actin, beta

Research Areas

Product Description	Host	Clonality	Application	Catalog# (PDF)
Anti-beta-Actin Antibody	Mouse	Monoclonal	WB,IP	100166-MM10

Documents
Datasheet Download
MSDS Download
Protocol Download
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