Anti-beta-Actin Antibody

Cat: 100166-T36
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Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-beta-Actin Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human beta-Actin
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IP 1-4 uL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Images
Human beta Actin/ACTB Immunoprecipitation(IP) 6113

ACTB was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-ACTB rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ACTB rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Human beta Actin/ACTB Western blot (WB) 6827

Anti-ACTB,beta-actin cytoplasmic1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: Mouse brain tissue lysate Whole Cell Lysate

Lane D: Mouse brain tissue lysate

Lane E: Rabbit brain tissue lysate

Lane F: Rabbite slpeen tissue lysate

Lane G: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:42 kDa

Anti-beta-Actin Antibody Alternative Names
Anti-BRWS1 Antibody;Anti-PS1TP5BP1 Antibody
beta-Actin Background Information
Full Name
actin, beta
Product Description Host Clonality Application Catalog# (PDF)
Anti-beta-Actin Antibody Mouse Monoclonal WB,IP 100166-MM10
info info
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