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beta Actin / ACTB 抗體, 兔多抗, 抗原親和純化

  • beta Actin / ACTB Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
  • beta Actin / ACTB Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
產品資料評論相關產品實驗方法
 Beta-Actin 抗體產品資訊
免疫原:A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB
Clone ID:
Ig類型:Rabbit IgG
濃度:
內毒素:
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB, and purified by antigen affinity chromatography.
 Beta-Actin Antibody WB Application Image
 Beta-Actin Antibody IP Application Image
Other Beta-Actin Antibody Products
Beta-Actin 研究背景

Beta-actin is an isform of actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. There are six different but highly conserved actin isoforms in vertebrates. Four of these isoforms are expressed primarily in striated and smooth muscle cells, whereas the two cytoplasmic β-actin and γ-actin isoforms are ubiquitously expressed. Conserved from birds to mammals, β-actin and γ-actin differ at only four biochemically similar amino acid residues. These amino acid differences confer unique biochemical properties between the two isoforms. Beta-actin is a major constituent of the contractile apparatus. Beta-actin, also known as a “housekeeping” protein, is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Loading controls are essential for proper interpretation of western blots. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.

 Beta-Actin 參考資料
  • Hanukoglu I., et al. 1983, J Mol Biol. 163(4): 673-8.
  • Mao B., et al. 2001, Nature. 411(6835): 321-5.
  • Holleran EA., et al. 2001, J Biol Chem. 276(39): 36598-605.
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