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Anti-RANKL Antibody

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Anti-RANKL Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-RANKL Antibody
Validated applications
WB,ELISA,ELISA(Det),IP
Species reactivity
Reacts with: Human
Specificity
Human RANKL
Immunogen
Recombinant Human RANKL / OPGL / TNFSF11 / CD254 protein (Catalog#11682-HNCH)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human RANKL / OPGL / TNFSF11 / CD254 (rh RANKL / OPGL / TNFSF11 / CD254; Catalog#11682-HNCH; O14788-2; Gly63-Asp244).
Source
Monoclonal Rabbit IgG Clone #101
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-RANKL Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 0.2-1 μL/mg of lysate
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Human RANKL / OPGL / TNFSF11 / CD254 in ELISA pair set (Catalog: # SEK11682). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11682-R128).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-RANKL Antibody (Rabbit Monoclonal antibody) Images

Anti-TNFSF11 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Membrane Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:38 kDa

TNFSF11 was immunoprecipitated using:

Lane A:0.5 mg Raji Whole Cell Lysate

0.5 µL anti-TNFSF11 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TNFSF11 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 40 kDa

Anti-RANKL Antibody: Alternative Names

Anti-CD254 Antibody; Anti-hRANKL2 Antibody; Anti-ODF Antibody; Anti-OPGL Antibody; Anti-OPTB2 Antibody; Anti-RANKL Antibody; Anti-sOdf Antibody; Anti-TRANCE Antibody

RANKL Background Information

Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.
Full Name
tumor necrosis factor (ligand) superfamily, member 11
References
  • Takayanagi H, et al. (2002) Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 4 Suppl 3: S227-32.
  • Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7.
  • Schwarz EM, et al. (2007) Clinical development of anti-RANKL therapy. Arthritis Res Ther. 9 Suppl 1: S7.
  • Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.

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