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Human CD28 Antibody

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Human CD28 Antibody ( antibody) General Information

Product name
Human CD28 Antibody
Validated applications
FCM,Func/A
Species reactivity
Reacts with: Human
Specificity
Human CD28
Source
Human IgG2 Clone #H001
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 0.5 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human CD28 Antibody ( antibody) Validated Applications

Application Dilution
FCM 0.5-2 μg/Test
Notes
Agonist Activity (Func/A): Agonist Activity – Measured by its ability to induce proliferation of human T cells. The ED50 for this effect is typically 0.2-1 ug/mL.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human CD28 Antibody ( antibody) Images

The staining effect of Human CD28 antibody to CD28 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis.
A: Lymphocytes were stained with PE-conjugated humanized CD28 antibody.
B: The binding effect of PE-conjugated human CD28 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD28 antibody (BD, 555725) for 20min (blue histograms).
CD28+ T cell staining of lymphocytes using human CD28 antibody by Flow cytometric analysis.
Lymphocytes were stained with PE-conjugated humanized CD28 antibody and CD19-APC (BD, 555415).
Effect of human CD28 antibody on stimulating IL-2 secretion of CD4 T cells co-activated with CD3 antibody. The isolated CD4+ T cells (1e5/well) were cultured in the 96-well cell plates pre-coated with 2 μg/mL human CD3 (humanized OKT3) antibody  (GMP-10977-H001) (100 μL/well) in the culture medium containing serial dilutions of CD28 antibody. 48 hours later, IL-2 secretion in the medium was measured by IL-2 Quantitative ELISA Kit.

Human CD28 Antibody: Alternative Names

Anti-Tp44 Antibody

CD28 Background Information

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Full Name
CD28 molecule
References
  • Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
  • Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
  • Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.
  • The role of Th1 / Th2 Cytokines played in Regulation of Specific CD4 + Th1 Cells Conversion and Activation during Inflammatory Reaction of Chronic Obstructive Pulmonary Disease
    Author
    Sun, J;Liu, T;Yan, Y;Huo, K;Zhang, W;Liu, H;Shi, Z;
    Year
    2018
    Journal
    Scand. J. Immunol.

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