ACVR2B Protein

ACVR2B Protein Overview

ACVR2B reagents

Activins are gonadal polypeptide hormones that are potent stimulators of FSH secretion and release in vitro (see 147380). Activins are dimers of 2 beta subunits that share extensive sequence homology with transforming growth factor-beta (190180). There are 2 classes of activin receptors, type I and type II (see ACVR2; 102581). By RT-PCR with primers based on the sequences of rodent type II activin receptor genes, Hilden et al. (1994) cloned an ACVR2B cDNA from the K562 erythroleukemic cell line. The predicted 512-amino acid protein, which they called ActRIIB, was 99% identical to the mouse homolog and 69% identical to ACVR2. ACVR2B contains an extracellular ligand binding domain, a transmembrane domain, and an intracellular serine/threonine kinase domain. Northern blot analysis of fetal tissues revealed that ACVR2B mRNAs are variably expressed in several tissues, with the highest level of expression in brain. On Northern blots of K562 cell mRNA, Hilden et al. (1994) found that ACVR2B was expressed predominantly as 2.5- and 10-kb transcripts, with a very weak signal at 2.1 kb.

ACVR2B protein family

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

ACVR2B protein name

Recommended name
Activin receptor type-2B
Aliases
ActR-IIB
Alternative name
Activin receptor type IIB ACTR-IIB

ACVR2B Gene family protein

Type 2 receptor serine/threonine kinases

ACVR2B Protein Sequence

Species Human ACVR2B protein
Length 512
Mass (Da) 57724
Sequence Human ACVR2B protein sequence
Species Mouse ACVR2B protein
Length 536
Mass (Da) 60542
Sequence Mouse ACVR2B protein sequence
Species Rat ACVR2B protein
Length 513
Mass (Da) 57857
Sequence Rat ACVR2B protein sequence

ACVR2B Protein Molecular Weight & PI

Activin receptor type-2B precursor (EC 2.7.11.30) (Activin receptor type IIB) (ACTR-IIB)Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 19-512 Activin receptor type-2B.

Molecular weight (Da)

55881.72

Theoretical pI

5.46

ACVR2B Protein Structure

Specificity and Structure of a high affinity Activin-like 1 (ALK1) signaling complex
Deposited
2012-05-22   Released:  2012-06-20
Deposition Author(s)
Townson, S.A., Martinez-Hackert, E., Greppi, C., Lowden, P., Sako, D., Liu, J., Ucran, J.A., Liharska, K., Underwood, K.W., Seehra, J., Kumar, R., Grinberg, A.V.
Organism(s)
Homo sapiens
Expression System
Cricetulus griseus
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.3570 Å
R-Value Free
0.261
R-Value Work
0.219
4FAO From PDB

Human ACVR2B protein Secondary structure

ACVR2B Protein Interaction

Recombinant ACVR2B Protein Feature

ACVR2B Protein, Human, Recombinant (Fc Tag)

High Purity
> 97 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL recombinant Activin A. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated human INHBA-His (Cat:10429-H08H), The EC50 of biotinylated human INHBA-His (Cat:10429-H08H) is 0.112 μg/mL. 4. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His (Cat:Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:Cat:50659-M08H) is 0.161 μg/mL.

Recombinant ACVR2B protein citations

Title
A validated, sensitive electrophoretic method for the detection of activin receptor type II-Fc fusion proteins in human blood
Year
2018
Author
Martin, L;Zouhiri, N;Audran, M;Marchand, A;
Journal
Drug Test Anal

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