UBASH3A Protein, Human, Recombinant (aa 354-623, His Tag)

1/1
Price:
Size:
Number:

UBASH3A Protein, Human, Recombinant (aa 354-623, His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mature form of human UBASH3A (AAH69511.1) (Ala354-Asn623) was expressed with a polyhistide tag at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human UBASH3A consists of 286 amino acids and predicts a molecular mass of 32.2 KDa. It migrates as an approximately 30 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

UBASH3A Protein, Human, Recombinant (aa 354-623, His Tag): Images

UBASH3A Protein, Human, Recombinant (aa 354-623, His Tag): Alternative Names

CLIP4 Protein, Human; STS-2 Protein, Human; TULA Protein, Human; TULA-1 Protein, Human

UBASH3A Background Information

UBASH3A is a member of the T-cell ubiquitin ligand (TULA) family. This family consists of two members. Both of them can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of UBASH3A gene results in multiple transcript variants. It interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. UBASH3A promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. UBASH3A also exhibits negligigle protein tyrosine phosphatase activity at neutral pH. It may act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. It may also inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
Full Name
ubiquitin associated and SH3 domain containing A
References
  • Collingwood TS, et al. (2007) T-cell ubiquitin ligand affects cell death through a functional interaction with apoptosis-inducing factor, a key factor of caspase-independent apoptosis.". J. Biol. Chem. 282 (42): 30920-8.
  • Smirnova EV, et al. (2008) TULA proteins bind to ABCE-1, a host factor of HIV-1 assembly, and inhibit HIV-1 biogenesis in a UBA-dependent fashion. Virology. 372(1):10-23.
  • Wattenhofer M, et al. (2001) Isolation and characterization of the UBASH3A gene on 21q22.3 encoding a potential nuclear protein with a novel combination of domains. Hum Genet. 108(2):140-7.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait