Thrombopoietin Protein, Mouse, Recombinant (His Tag)

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Thrombopoietin Protein, Mouse, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL.
Protein Construction
A DNA sequence encoding the mouse THPO (NP_033405.1) precursor (Met 1-Thr 356) with a N-terminal polyhistidine tag was expressed.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Ser 22
Molecule Mass
The recombinant mouse THPO consists of 346 amino acids with the predicted molecular mass of 37 kDa. As a result of glycosylation, rmTHPO migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Thrombopoietin Protein, Mouse, Recombinant (His Tag): Images

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL.

Thrombopoietin Protein, Mouse, Recombinant (His Tag): Alternative Names

Mgdf Protein, Mouse; Ml Protein, Mouse; Mpllg Protein, Mouse; Tpo Protein, Mouse

Thrombopoietin Background Information

Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.
Full Name
thrombopoietin
References
  • Ryu T,et al.(2003) Thrombopoietin-producing hepatocellular carcinoma. Intern Med. 42(8): 730-4.
  • Higashihara M,et al. (2003) Thrombopoietin-producing tumor. Intern Med. 42(8): 632-3?
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