TRAIL Protein, Human, Recombinant

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TRAIL Protein, Human, Recombinant: Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human TNFSF10 (NP_003801.1) (Val 114-Gly 281) with an initial Met was expressed and purified.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human TNFSF10 consisting of 169 amino acids and has a calculated molecular mass of 19.6 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 40mM Tris, 0.3 M NaCl, pH 7.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TRAIL Protein, Human, Recombinant: Images

TRAIL Protein, Human, Recombinant: Alternative Names

Apo-2L Protein, Human; APO2L Protein, Human; CD253 Protein, Human; TL2 Protein, Human; TRAIL Protein, Human

TRAIL Background Information

Tumor necrosis factor ligand superfamily member 1 (TNFSF1), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF1 / Apo-2L / CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF1 / TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF1 / CD253 is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF1 / Apo-2L / CD253 / TRAIL binds to several members of TNF receptor superfamily including TNFRSF1A / TRAILR1, TNFRSF1B / TRAILR2, TNFRSF1C / TRAILR3, TNFRSF1D / TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF1 / TRAIL may be modulated by binding to the decoy receptors TNFRSF1C / TRAILR3, TNFRSF1D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8 / JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Full Name
tumor necrosis factor (ligand) superfamily, member 10
References
  • Song C, et al. (2005) TRAIL (CD253), a new member of the TNF superfamily. J Biol Regul Homeost Agents. 19(1-2): 73-7.
  • Kuribayashi K, et al. (2008) TNFSF10 (TRAIL), a p53 target gene that mediates p53-dependent cell death. Cancer Biol Ther. 7(12): 2034-8.
  • Wiley SR, et al. (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 3(6): 673-82.
  • Preclinical studies of a death receptor 5 fusion protein that ameliorates acute liver failure
    Author
    Chen, Q;Wang, P;Zhang, Q;Xia, M;Zhang, G;Li, J;Shen, E;Chen, YH;Wan, X;
    Year
    2019
    Journal
    J. Mol. Med.
    Application
    SPR
  • Treatment of acetaminophen-induced liver failure by blocking the death checkpoint protein TRAIL
    Author
    Chen, Q;Yan, D;Zhang, Q;Zhang, G;Xia, M;Li, J;Zhan, W;Shen, E;Li, Z;Lin, L;Chen, Y;Wan, X;
    Year
    2019
    Journal
    Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
    Application
    cell-based
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