|Recombinant Human TNF-alpha / TNFA protein (Catalog#10602-HNAE)|
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|0.2 μm filtered solution in PBS|
|This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha / TNFA (rHuman TNF-alpha / TNFA; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233) and was produced using recombinant antibody technology.|
Has cross-reactivity with Cynomolgus TNFa (Catalog # 90330-CNAE) /Canine TNFa Catalog # (70003-DNAE) and Ferret TNFa (Catalog # 60002-FNAE)
No cross-reactivity with Mouse TNFa (Catalog # 50349-MNAE ) and Rat TNFa (Catalog # 80045-RNAE) in ELISA assay
In a functional ELISA which immobilized recombinant Human TNFR1 (Catalog # 10872-H08H) at 1μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human TNFα Monoclonal Antibody (Catalog # 10602-R10N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog # 10602-HNAE) ) to human TNFR1,the EC50 is 1.25 μg/mL;In a functional ELISA which immobilized recombinant Human TNFR2 (Catalog # 10417-H08H) at 2 μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human TNFα Monoclonal Antibody (Catalog # 10602-R10N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog # 10602-HNAE) ) to human TNFR1,the EC50 is 1.26 μg/mL.
The neutralization activity of TNFa Neutralizing Antibody is Measured by its ability to neutralize TNF-α induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 8.8-35.2 ng/mL in the presence of 2 ng/mL Recombinant Human TNF-α (10602-HNAE) and 1 μg/mL actinomycin D.
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.