Reacts with: Human
Has cross-reactivity with Cynomolgus TNFa (Catalog # 90330-CNAE) /Canine TNFa Catalog # (70003-DNAE) and Ferret TNFa (Catalog # 60002-FNAE)
No cross-reactivity with Mouse TNFa (Catalog # 50349-MNAE ) and Rat TNFa (Catalog # 80045-RNAE) in ELISA assay
Recombinant Human TNF-alpha / TNFA protein (Catalog#10602-HNAE)
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha / TNFA (rHuman TNF-alpha / TNFA; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233) and was produced using recombinant antibody technology.
Monoclonal Rabbit IgG Clone #R101
0.2 μm filtered solution in PBS
Please contact us for more information.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Flow cytometric analysis of Human TNF-α expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human TNF-α, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Cytotoxicity Induced by TNFα was Neutralized by Human TNFα Antibody. Recombinant Human TNFα (Catalog 10602-HNAE) induces cytotoxicity in the the L929 mouse fibroblast cell line. Cytotoxicity elicited by 2 ng/mL of Recombinant Human TNFα is neutralized by increasing concentrations of Anti Human TNFα neutralizing Rabbit Antibody (Catalog # 10602-R101). The IC50 is typically 8.8-35.2 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).
Anti-DIF Antibody;Anti-TNF-alpha Antibody;Anti-TNFA Antibody;Anti-TNFSF2 Antibody
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
TNF-alpha Protein & Antibody
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5. Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.