Anti-TNF-alpha Antibody

Cat: 10602-R10N1
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All TNF-alpha Reagents
Anti-TNF-alpha Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-TNF-alpha Antibody
Validated applications
FCM,Neutralization
Species reactivity
Reacts with: Human
Specificity
Human TNF-alpha
Has cross-reactivity with Cynomolgus TNFa (Catalog # 90330-CNAE) /Canine TNFa Catalog # (70003-DNAE) and Ferret TNFa (Catalog # 60002-FNAE)
No cross-reactivity with Mouse TNFa (Catalog # 50349-MNAE ) and Rat TNFa (Catalog # 80045-RNAE) in ELISA assay
Immunogen
Recombinant Human TNF-alpha / TNFA protein (Catalog#10602-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNF-alpha / TNFA (rHuman TNF-alpha / TNFA; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #R101
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TNF-alpha Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
FCM 0.5-2 μg/Test  
Application Notes
Block In a functional ELISA which immobilized recombinant Human TNFR1 (Catalog # 10872-H08H) at 1μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human TNFα Monoclonal Antibody (Catalog # 10602-R10N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog # 10602-HNAE) ) to human TNFR1,the EC50 is 1.25 μg/mL;In a functional ELISA which immobilized recombinant Human TNFR2 (Catalog # 10417-H08H) at 2 μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human TNFα Monoclonal Antibody (Catalog # 10602-R10N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog # 10602-HNAE) ) to human TNFR1,the EC50 is 1.26 μg/mL.
Neutralization The neutralization activity of TNFa Neutralizing Antibody is Measured by its ability to neutralize TNF-α induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 8.8-35.2 ng/mL in the presence of 2 ng/mL Recombinant Human TNF-α (10602-HNAE) and 1 μg/mL actinomycin D.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TNF-alpha Antibody (Rabbit Monoclonal antibody) Images
Human TNF-alpha/TNFA/TNFSF2 Flow Cytometry (FC) 5002
Flow cytometric analysis of Human TNF-α expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human TNF-α, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Human TNF-alpha/TNFA/TNFSF2 Blocking/Neutralization (B/N) 5584
Cytotoxicity Induced by TNFα was Neutralized by Human TNFα Antibody. Recombinant Human TNFα (Catalog 10602-HNAE) induces cytotoxicity in the the L929 mouse fibroblast cell line. Cytotoxicity elicited by 2 ng/mL of Recombinant Human TNFα is neutralized by increasing concentrations of Anti Human TNFα neutralizing Rabbit Antibody (Catalog # 10602-R101). The IC50 is typically 8.8-35.2 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).
Anti-TNF-alpha Antibody Alternative Names
Anti-DIF Antibody;Anti-TNF-alpha Antibody;Anti-TNFA Antibody;Anti-TNFSF2 Antibody
TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 70003-R001
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 60002-R030
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 60002-R303
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 90330-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 70003-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-T16
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 60002-MM11
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 60002-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80045-T50
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 80045-T16
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-T16
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 50349-R009
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA(Cap) 50349-R023
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 50349-RP02
    Anti-TNF-alpha Antibody Rabbit Monoclonal Neutralization 50349-RN023
    Anti-TNF-alpha Antibody Mouse Monoclonal Neutralization 10602-MM0N1
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA,IP 10602-RP02
    Anti-TNF-alpha Antibody Rabbit Polyclonal WB,ELISA 10602-RP03
    Anti-TNF-alpha Antibody (APC) Rabbit Monoclonal FCM 10602-R101-A
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Det) 10602-MM08
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-R211
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-RP01
    Anti-TNF-alpha Antibody (PE) Rabbit Monoclonal FCM 10602-R101-P
    Anti-TNF-alpha Antibody Mouse Monoclonal ELISA,ELISA(Cap),FCM 10602-MM01
    Anti-TNF-alpha Antibody Rabbit Polyclonal ELISA 10602-T16
    Anti-TNF-alpha Antibody Rabbit Monoclonal ELISA 10602-RE23
    Anti-TNF-alpha Antibody (FITC) Rabbit Monoclonal FCM 10602-R101-F
    Anti-TNF-alpha Antibody Rabbit Monoclonal FCM 10602-R101
    Anti-TNF-alpha Antibody (FITC) Mouse Monoclonal FCM 10602-MM01-F
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