Anti-Smad5 Antibody

Cat: 50728-RP02

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All Smad5 Reagents

Anti-Smad5 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Smad5 Antibody
Validated applications
Species reactivity
Reacts with: Mouse
Mouse Smad5
Recombinant Mouse SMAD5 protein (Catalog#50728-M20B)
Produced in rabbits immunized with purified, recombinant Mouse SMAD5 (rM SMAD5; Catalog#50728-M20B; P97454; Thr2-Ser465). SMAD5 specific IgG was purified by Mouse SMAD5 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-Smad5 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Smad5 Antibody (Rabbit Polyclonal antibody) Images

Mouse Smad5 Immunohistochemistry(IHC) 3176
Immunochemical staining of mouse SMAD5 in mouse intestine with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-Smad5 Antibody Alternative Names

Anti-1110051M15Rik Antibody;Anti-AI451355 Antibody;Anti-Dwf-C Antibody;Anti-Madh5 Antibody;Anti-MusMLP Antibody

Smad5 Background Information

SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Full Name
SMAD family member 5
  • Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8.
  • Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23.
  • Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Smad5 Antibody Rabbit Polyclonal ELISA 50728-RP01
    Anti-Smad5 Antibody Rabbit Polyclonal WB,IHC-P,ICC/IF,IF 101483-T42
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