S100A3 Protein, Mouse, Recombinant (His Tag)

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S100A3 Protein, Mouse, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mouse S100A3 (NP_035440.1) (Met1-Gln101) was expressed with a polyhistidine tag at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
His
Molecule Mass
The recombinant mouse S100A3 consists of 119 amino acids and predicts a molecular mass of 14 kDa. It migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A3 Protein, Mouse, Recombinant (His Tag): Images

S100A3 Protein, Mouse, Recombinant (His Tag): Alternative Names

S100E Protein, Mouse

S100A3 Background Information

Protein S1-A3, also known as Protein S-1E, S1 calcium-binding protein A3, S1A3 and S1E, is a member of the S-1 family. S1A3 / S1E contains 2EF-hand domains. S1A3 / S1E is highly expressed in the differentiating cuticular cells within the hair follicle and organized into mature hair cuticles. High concentrations of S1A3 homotetramer might provide the millimolar level of Ca2+ required for hair cuticular barrier formation. S1A3 / S1E is a unique member of the Ca2+-binding S1 protein family with the highest cysteine content and affinity for Zn2+. S1A3 / S1E binds both calcium and zinc. S1A3 / S1E probably binds 2 zinc ions per molecule. It may be involved in calcium-dependent cuticle cell differentiation and hair shaft formation. S1A3 plays an important role in calcium-dependent processes leading to hair shaft formation. S1A3 / S1E is a unique protein among all members of the calcium-binding S1 family, is specifically expressed at the inner endocuticle of human hair fibers. Upon hair damage, S1A3 / S1E is released from hair fibers and possibly destabilizes the hair tissue architecture.
Full Name
S100 calcium binding protein A3
References
  • Kizawa, K. et al., 2008, J Biol Chem. 283 (8):5004-13.
  • Kizawa, K. et al., 1998, J Invest Dermatol. 111 (5):879-86.
  • Kizawa, K. et al., 2002, Biochem Biophys Res Commun. 299 (5):857-62.
  • Fritz,G. et al., 2002, J Biol Chem. 277 (36):33092-8.
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