PHYH qPCR Primer Pairs, Rat

Cat: RP300645
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PHYH qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
PHYH Background Information

PHYH belongs to the family of iron(II)-dependent oxygenases, which typically incorporate one atom of dioxygen into the substrate and one atom into the succinate carboxylate group. PHYH is expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle. It converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Defects in PHYH can cause Refsum disease (RD). RD is an autosomal recessive disorder characterized clinically by a tetrad of abnormalities: retinitis pigmentosa, peripheral neuropathy, cerebellar ataxia, and elevated protein levels in the cerebrospinal fluid (CSF). Patients exhibit accumulation of the branched-chain fatty acid, phytanic acid, in blood and tissues.

Full Name
phytanoyl-CoA 2-hydroxylase
References
  • Mihalik SJ, et al. (1997) Identification of PAHX, a Refsum disease gene. Nat Genet. 17(2): 185-9.
  • McDonough MA, et al. (2005) Structure of human phytanoyl-CoA 2-hydroxylase identifies molecular mechanisms of Refsum disease. J Biol Chem. 280(49):41101-10.
  • Jansen GA, et al. (1998) Characterization of phytanoyl-Coenzyme A hydroxylase in human liver and activity measurements in patients with peroxisomal disorders. Clin Chim Acta. 271 (2):203-11.
  • PHYH qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    PHYH cDNA ORF Clone in Cloning Vector, Rat RG80681-U
    PHYH cDNA ORF Clone, Rat, untagged RG80681-UT
    PHYH Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80681-ACGLN
    PHYH cDNA ORF Clone, Rat, C-GFPSpark® tag RG80681-ACG
    PHYH cDNA ORF Clone, Rat, N-GFPSpark® tag RG80681-ANG
    PHYH cDNA ORF Clone, Rat, C-OFPSpark® tag RG80681-ACR
    PHYH cDNA ORF Clone, Rat, N-OFPSpark® tag RG80681-ANR
    PHYH cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80681-CF
    PHYH cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80681-NF
    PHYH cDNA ORF Clone, Rat, C-His tag RG80681-CH
    PHYH cDNA ORF Clone, Rat, N-His tag RG80681-NH
    PHYH cDNA ORF Clone, Rat, C-HA tag RG80681-CY
    PHYH cDNA ORF Clone, Rat, N-HA tag RG80681-NY
    PHYH cDNA ORF Clone, Rat, C-Myc tag RG80681-CM
    PHYH cDNA ORF Clone, Rat, N-Myc tag RG80681-NM

    PHYH qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    PHYH cDNA ORF Clone in Cloning Vector, Mouse MG5A0786-U
    PHYH cDNA ORF Clone, Mouse, untagged MG5A0786-UT
    PHYH Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG5A0786-ACGLN
    PHYH cDNA ORF Clone, Mouse, C-GFPSpark® tag MG5A0786-ACG
    PHYH cDNA ORF Clone, Mouse, N-GFPSpark® tag MG5A0786-ANG
    PHYH cDNA ORF Clone, Mouse, C-OFPSpark® tag MG5A0786-ACR
    PHYH cDNA ORF Clone, Mouse, N-OFPSpark® tag MG5A0786-ANR
    PHYH cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG5A0786-CF
    PHYH cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG5A0786-NF
    PHYH cDNA ORF Clone, Mouse, C-His tag MG5A0786-CH
    PHYH cDNA ORF Clone, Mouse, N-His tag MG5A0786-NH
    PHYH cDNA ORF Clone, Mouse, C-HA tag MG5A0786-CY
    PHYH cDNA ORF Clone, Mouse, N-HA tag MG5A0786-NY
    PHYH cDNA ORF Clone, Mouse, C-Myc tag MG5A0786-CM
    PHYH cDNA ORF Clone, Mouse, N-Myc tag MG5A0786-NM

    PHYH qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    PHYH cDNA ORF Clone in Cloning Vector, Human HG13368-G
    PHYH cDNA ORF Clone, Human, untagged HG13368-UT
    PHYH cDNA ORF Clone, Human, C-GFPSpark® tag HG13368-ACG
    PHYH Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13368-ACGLN
    PHYH cDNA ORF Clone, Human, N-GFPSpark® tag HG13368-ANG
    PHYH cDNA ORF Clone, Human, C-OFPSpark® tag HG13368-ACR
    PHYH cDNA ORF Clone, Human, N-OFPSpark® tag HG13368-ANR
    PHYH cDNA ORF Clone, Human, C-DDK (Flag®) tag HG13368-CF
    PHYH cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13368-NF
    PHYH cDNA ORF Clone, Human, C-His tag HG13368-CH
    PHYH cDNA ORF Clone, Human, N-His tag HG13368-NH
    PHYH cDNA ORF Clone, Human, C-HA tag HG13368-CY
    PHYH cDNA ORF Clone, Human, N-HA tag HG13368-NY
    PHYH cDNA ORF Clone, Human, C-Myc tag HG13368-CM
    PHYH cDNA ORF Clone, Human, N-Myc tag HG13368-NM
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