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大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤

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    大鼠 NUDT5 cDNA Clone產品資訊
    註冊碼:NM_001007733.1
    cDNA基因長度:660bp
    cDNA基因描述:Full length Clone DNA of Rattus norvegicus nudix (nucleoside diphosphate linked moiety X)-type motif 5 with C terminal Flag tag.
    基因別名:Nudt5
    分子種屬:Rat
    載體:pCMV3-C-FLAG
    Plasmid:
    限制性酶切位點:
    Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with NUDT5 qPCR primers for gene expression analysis, RP301066 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤 on other vectors
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤RG81109-ACGNT$8150
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤RG81109-ACRNT$8150
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤RG81109-ANGNT$8150
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤RG81109-ANRNT$8150
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤RG81109-CFNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-His 標籤RG81109-CHNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤RG81109-CMNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤RG81109-CYNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤RG81109-NFNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), N-His 標籤RG81109-NHNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤RG81109-NMNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤RG81109-NYNT$7060
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF(克隆載體)RG81109-UNT$2720
    大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因全長cDNA ORF克隆 (表達載體)RG81109-UTNT$7060
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    研究背景

    ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.

    參考資料
  • Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101.
  • Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.
  • Size / Price
    貨號: RG81109-CF
    目錄價: 
    單價:      (You Save: )

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