|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80459-ACG|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80459-ACR|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80459-CF|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80459-CH|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80459-CM|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80459-CY|
|大鼠 IL-9 基因全長cDNA ORF(克隆載體)||RG80459-G|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80459-NF|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80459-NH|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80459-NM|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80459-NY|
|大鼠 IL-9 基因全長cDNA ORF克隆 (表達載體)||RG80459-UT|
Interleukin 9, also known as IL-9, is a cytokine (cell signalling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells, synergizes with TGF-β1 to differentiate naïve CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight a role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.