( We provide with IL2RG qPCR primers for gene expression analysis, RP300189 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80197-ACG|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80197-ACR|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80197-CF|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80197-CH|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80197-CM|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80197-CY|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF(克隆載體)||RG80197-G|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80197-G-F|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80197-NF|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80197-NH|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80197-NM|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80197-NY|
|大鼠 CD132 / IL2RG 基因全長cDNA ORF克隆 (表達載體)||RG80197-UT|
The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (XSCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor on the basis of chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.