|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80187-ACG|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80187-ACR|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80187-CF|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80187-CH|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80187-CM|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80187-CY|
|大鼠 IL20RB 基因全長cDNA ORF(克隆載體)||RG80187-G|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80187-NF|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80187-NH|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80187-NM|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80187-NY|
|大鼠 IL20RB 基因全長cDNA ORF克隆 (表達載體)||RG80187-UT|
IL20RB belongs to the type II cytokine receptor family. There are two kinds of type II cytokine receptors : cytokine receptors that bind type I and type II interferons; cytokine receptors that bind members of the interleukin-10 family (interleukin-10, interleukin-20 and interleukin-22). Type II cytokine receptors are similar to type I cytokine receptors except they do not possess the signature sequence WSXWS that is characteristic of type I receptors. They are expressed on the surface of certain cells, which bind and respond to a select group of cytokines. These receptors are related predominantly by sequence similarities in their extracellular portions that are composed of tandem Ig-like domains. The intracellular domain of type II cytokine receptors is typically associated with a tyrosine kinase belonging to the Janus kinase (JAK) family. IL20RB and IL20RA (MIM 605620) form a heterodimeric receptor for interleukin-20.