|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80185-ACG|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80185-ACR|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80185-CF|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80185-CH|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80185-CM|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80185-CY|
|大鼠 IL12RB2 基因全長cDNA ORF(克隆載體)||RG80185-G|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80185-NF|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80185-NH|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80185-NM|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80185-NY|
|大鼠 IL12RB2 基因全長cDNA ORF克隆 (表達載體)||RG80185-UT|
Interleukin-12 receptor subunit beta-2 (IL12RB2), also known as IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, and IL-12RB2, is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. IL12RB2 belongs to the type I cytokine receptor family. The coexpression of IL12RB2 and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of IL12RB2 is up-regulated by IFN gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of IL12RB2 is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. IL12RB2 promotes the proliferation of T-cells as well as NK cells. IL12RB2 induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.