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大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

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Rat GOT1 cDNA Clone產品資訊
註冊碼:NM_012571.2
cDNA基因長度:1242bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) with N terminal HA tag.
基因別名:cCAT, Aspat, Gaspat, cAspAT
分子種屬:Rat
載體:pCMV3-N-HA
Plasmid:
限制性酶切位點:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤 on other vectors
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤RG80493-ACGNT$8150
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤RG80493-ACRNT$8150
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤RG80493-ANGNT$8150
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤RG80493-ANRNT$8150
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤RG80493-CFNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤RG80493-CHNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤RG80493-CMNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤RG80493-CYNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤RG80493-NFNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤RG80493-NHNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤RG80493-NMNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤RG80493-NYNT$7060
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF(克隆載體)RG80493-UNT$2720
大鼠 Aspartate aminotransferase / GOT1 基因全長cDNA ORF克隆 (表達載體)RG80493-UTNT$7060
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研究背景

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

參考資料
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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    貨號: RG80493-NY
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