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大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤

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Rat GLRX cDNA Clone產品資訊
註冊碼:NM_022278.1
cDNA基因長度:324bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus glutaredoxin (thioltransferase) with C terminal Flag tag.
基因別名:Grx, Glrx1
分子種屬:Rat
載體:pCMV3-C-FLAG
Plasmid:
限制性酶切位點:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤 on other vectors
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤RG81101-ACGNT$8150
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤RG81101-ACRNT$8150
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤RG81101-ANGNT$8150
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤RG81101-ANRNT$8150
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤RG81101-CFNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-His 標籤RG81101-CHNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤RG81101-CMNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤RG81101-CYNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤RG81101-NFNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-His 標籤RG81101-NHNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤RG81101-NMNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤RG81101-NYNT$7060
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF(克隆載體)RG81101-UNT$2720
大鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體)RG81101-UTNT$7060
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研究背景

Glutaredoxin-1, also known as GRX1 and GLRX, belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

參考資料
  • Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97.
  • Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6.
  • Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.
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    貨號: RG81101-CF
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