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大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

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    大鼠 FTL cDNA Clone產品資訊
    註冊碼:BC061525.1
    cDNA基因長度:552bp
    cDNA基因描述:Full length Clone DNA of Rattus norvegicus ferritin, light polypeptide with N terminal HA tag.
    基因別名:Ftl1
    分子種屬:Rat
    載體:pCMV3-N-HA
    Plasmid:
    限制性酶切位點:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with FTL qPCR primers for gene expression analysis, RP300455 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤 on other vectors
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤RG80491-ACGNT$8150
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    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤RG80491-ANGNT$8150
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤RG80491-ANRNT$8150
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤RG80491-CFNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), C-His 標籤RG80491-CHNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤RG80491-CMNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤RG80491-CYNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤RG80491-NFNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-His 標籤RG80491-NHNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤RG80491-NMNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤RG80491-NYNT$7060
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF(克隆載體)RG80491-UNT$2720
    大鼠 FTL/ferritin, light polypeptide 基因全長cDNA ORF克隆 (表達載體)RG80491-UTNT$7060
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    研究背景

    Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.

    參考資料
  • Munro HN, et al. (1988) The ferritin genes: structure, expression, and regulation. Ann N Y Acad Sci. 526: 113-23.
  • Zhang Y, et al. (2008) Comparative proteomic analysis of human placenta derived from assisted reproductive technology. Proteomics. 8 (20): 4344-56.
  • Lebo RV, et al. (1986) Human ferritin light chain gene sequences mapped to several sorted chromosomes. Hum Genet. 71 (4): 325-8.
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    貨號: RG80491-NY
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    Datasheet & Documentation

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