|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG81138-ACG|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG81138-ACR|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||RG81138-ANG|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||RG81138-ANR|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG81138-CF|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG81138-CH|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG81138-CM|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG81138-CY|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG81138-NF|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG81138-NH|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG81138-NM|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG81138-NY|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF(克隆載體)||RG81138-U|
|大鼠 FKBP12/FKBP1A 基因全長cDNA ORF克隆 (表達載體)||RG81138-UT|
FK506 binding protein 12 (FKBP12), also known as FKBP1, along with cyclophilin, are two major members of the immunophilin protein family who serve as receptors for the immunosuppressant drugs cyclosporin A and FK506. As a conserved molecules in many eukaryotes, FKBP12 has been characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues, and is involved in several biochemical processes including protein folding, receptor signaling, protein trafficking and transcription. FKBP12 has attracted immense attention and its role in mediating the immunosuppressive functions. FKBP12 serves a dual role as a peptidyl-prolyl cis-trans isomerase and as a modulator of several cell signaling pathways. In one such a role, FKBP12 interacts with and regulates the functional state of the ryanodine Ca2+ channel receptor by altering protein conformation and coordinating multi-protein complex formation. Another physiological role of FKBP12 is an interactor and a regulator of the type I serine/threonine kinase receptors of TGF-beta superfamily. Current data, derived from detailed biochemical studies as well as from functional studies in various systems, suggest that FKBP12 functions as a "guardian" for the type I receptors to prevent them from leaky signaling under sub-optimal ligand concentrations, thereby providing a molecular "gradient reader" for TGF-beta family morphogens. This aspect of FKBP12 function may be critical for cellular responsiveness to morphogenetic gradients of the TGF-beta family members during early development, serving to assure the translation of different ligand concentrations into different signaling readouts. In addition, FKBP12 may be involved in neuronal or astrocytic cytoskeletal organization and in the abnormal metabolism of tau protein in Alzheimer's disease (AD) damaged neurons.