|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80163-ACG|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80163-ACR|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80163-CF|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80163-CH|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80163-CM|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80163-CY|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF(克隆載體)||RG80163-G|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80163-NF|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80163-NH|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80163-NM|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80163-NY|
|大鼠 Fractalkine/CX3CL1 基因全長cDNA ORF克隆 (表達載體)||RG80163-UT|
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.