|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80443-ACG|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80443-ACR|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80443-CF|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80443-CH|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80443-CM|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80443-CY|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF(克隆載體)||RG80443-G|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80443-NF|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80443-NH|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80443-NM|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80443-NY|
|大鼠 Cathepsin S/CTSS 基因全長cDNA ORF克隆 (表達載體)||RG80443-UT|
Cathepsin S (CTSS), one of the lysosomal proteinases, has many important physiological functions in the nervous system, especially in process of extracellular matrix degradation and endocellular antigen presentation. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. Cathepsin S is expressed in the lysosome of antigen presenting cells, primarily dendritic cells, B-cells and macrophages. Compared with other lysosomal cysteine proteases, cathepsin S has displayed some unique characteristics. Cathepsin S is most well known for its critical function in the proteolytic digestion of the invariant chain chaperone molecules, thus controlling antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules or to NK1.1+ T-cells via CD1 molecules. Cathepsin S also appears to participate in direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells, or in cross-presentation by MHC class I molecules to CD8+ T-cells. In addition, although direct evidence is still lacking, in its secreted form cathepsin S is implicated in degradation of the extracellular matrix, which may contribute to the pathology of a number of diseases, including arthritis, atherosclerosis, neurological diseases and chronic obstructive pulmonary disease.