|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG81328-ACG|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG81328-ACR|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG81328-CF|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG81328-CH|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG81328-CM|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG81328-CY|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG81328-NF|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG81328-NH|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG81328-NM|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG81328-NY|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF(克隆載體)||RG81328-U|
|大鼠 Cathepsin D/CTSD 基因全長cDNA ORF克隆 (表達載體)||RG81328-UT|
Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.