|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat CNTN3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80316-ACG|
|Rat CNTN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80316-ACR|
|Rat CNTN3 ORF mammalian expression plasmid, C-Flag tag||RG80316-CF|
|Rat CNTN3 ORF mammalian expression plasmid, C-His tag||RG80316-CH|
|Rat CNTN3 ORF mammalian expression plasmid, C-Myc tag||RG80316-CM|
|Rat CNTN3 ORF mammalian expression plasmid, C-HA tag||RG80316-CY|
|Rat CNTN3 Gene cDNA clone plasmid||RG80316-G|
|Rat CNTN3 ORF mammalian expression plasmid, N-Flag tag||RG80316-NF|
|Rat CNTN3 ORF mammalian expression plasmid, N-His tag||RG80316-NH|
|Rat CNTN3 ORF mammalian expression plasmid, N-Myc tag||RG80316-NM|
|Rat CNTN3 ORF mammalian expression plasmid, N-HA tag||RG80316-NY|
|Rat CNTN3 natural ORF mammalian expression plasmid||RG80316-UT|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.