( We provide with CD55 qPCR primers for gene expression analysis, RP300290 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80317-ACG|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80317-ACR|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80317-CF|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80317-CH|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80317-CM|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80317-CY|
|大鼠 CD55/DAF 基因全長cDNA ORF(克隆載體)||RG80317-G|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80317-NF|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80317-NH|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80317-NM|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80317-NY|
|大鼠 CD55/DAF 基因全長cDNA ORF克隆 (表達載體)||RG80317-UT|
CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.