|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80721-ACG|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80721-ACR|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80721-CF|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80721-CH|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80721-CM|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80721-CY|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80721-NF|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80721-NH|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80721-NM|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80721-NY|
|大鼠 CCL6/C10 基因全長cDNA ORF(克隆載體)||RG80721-U|
|大鼠 CCL6/C10 基因全長cDNA ORF克隆 (表達載體)||RG80721-UT|
Chemokine (C-C motif) ligand 6 (CCL6), also known as C-C chemokine C10 has only been identified in rodents, which is a small cytokine belonging to the CC chemokine family, beta-chemokine subfamily. C-C chemokine C10 is involved in the chronic stages of host defense reactions. C10 chemokine rapidly promotes disease resolution in the cecal ligation and puncture (CLP) model through its direct effects on the cellular events critically involved in host defense during septic peritonitis. CCL6 appears to contribute to the macrophage infiltration that is independent of other CC chemokines. C10 is a prominent chemokine expressed in the central nervous system in experimental inflammatory demyelinating disease, also acts as a potent chemotactic factor for the migration of these leukocytes to the brain. CCL6 may be a mediator released by microglia for cell-cell communication under physiological as well as pathological conditions of CNS. Additionally, the chemokine CCL6 may alter tumor behavior by relieving its growth factor dependency and by promoting invasiveness as a result of local tissue apoptosis.