|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG81126-ACG|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG81126-ACR|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG81126-CF|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG81126-CH|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG81126-CM|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG81126-CY|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG81126-NF|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG81126-NH|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG81126-NM|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG81126-NY|
|大鼠 Calreticulin 基因全長cDNA ORF(克隆載體)||RG81126-U|
|大鼠 Calreticulin 基因全長cDNA ORF克隆 (表達載體)||RG81126-UT|
Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.