( We provide with AHSG qPCR primers for gene expression analysis, RP300433 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||RG80469-ACG|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||RG80469-ACR|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||RG80469-CF|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||RG80469-CH|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||RG80469-CM|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||RG80469-CY|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||RG80469-NF|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||RG80469-NH|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||RG80469-NM|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||RG80469-NY|
|大鼠 Fetuin-A 基因全長cDNA ORF(克隆載體)||RG80469-U|
|大鼠 Fetuin-A 基因全長cDNA ORF克隆 (表達載體)||RG80469-UT|
Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.