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大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤

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Rat ADSL cDNA Clone產品資訊
註冊碼:NM_001130503.1
cDNA基因長度:1455bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus adenylosuccinate lyase with N terminal Flag tag.
基因別名:null
分子種屬:Rat
載體:pCMV3-N-FLAG
Plasmid:
限制性酶切位點:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤 on other vectors
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤RG81324-ACGNT$8151
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤RG81324-ACRNT$7440
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤RG81324-ANGNT$7440
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤RG81324-ANRNT$7440
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤RG81324-CFNT$7064
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤RG81324-CHNT$6450
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤RG81324-CMNT$7064
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤RG81324-CYNT$6450
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF(克隆載體)RG81324-GNT$2717
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤RG81324-NFNT$7064
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤RG81324-NHNT$6450
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤RG81324-NMNT$6450
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤RG81324-NYNT$6450
大鼠 ADSL / Adenylosuccinate Lyase 基因全長cDNA ORF克隆 (表達載體)RG81324-UTNT$6450
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Product nameProduct name
研究背景

Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.

參考資料
  • Nassogne M, et al. (2000) Adenylosuccinase deficiency: an unusual cause of early-onset epilepsy associated with acquired microcephaly. Brain and development. 22 (6): 383-6.
  • Sivendran S, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. J Biol Chem. 279 (51): 53789-97.
  • Lee TT, et al. (1999) His68 and His141 are critical contributors to the intersubunit catalytic site of adenylosuccinate lyase of Bacillus subtilis. Biochemistry. 38 (1): 22-32.
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    貨號: RG81324-NF
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